Many circumstances can also be influence recombination inside D. melanogaster and additionally lady years, temperature, level of matings or dinner –. To minimize the effects of those items, all the crosses and you can strains was basically was able at the same heat (23C) lower than a good a dozen hours. light/dark stage, that have ongoing travel occurrence in half pint bottle into basic corn-buffet media. Ergo all crosses was basically carried out with flies of similar age, which have 6 hours dated virgin flies allowed to lover and you may set eggs getting a time period of thirty-six–48 hours, right after which the parents was basically thrown away as well as the offspring was in fact allowed so you’re able to arise. (więcej…)